Nuclear polyhedrosis viruses of insects.
نویسندگان
چکیده
منابع مشابه
Plaque assay of nuclear polyhedrosis viruses in cell culture.
The nuclear polyhedrosis virus of Autographa californica has been titrated in Spodoptera frugiperda cells by the plaque method, using a solid overlay which does not require either the use of modified culture medium or expensive purified agarose or the addition of culture medium as a liquid layer above the solid agarose. This assay is more sensitive than that using a viscous methyl cellulose ove...
متن کاملPlant Viruses and Insects
The principal families of insect vectors which cause the most damage to agricultural crops through the spread of plant diseases are in the order Hemiptera, and include the aphids, leafhoppers, delphacid planthoppers and whiteflies. Another important group of insect vectors found worldwide is the order Thysanoptera, the thrips. Other insects also spread plant diseases; however, aphids alone are ...
متن کاملCharacterization of nuclear polyhedrosis virus DNAs.
The nuclear polyhedrosis virus DNAs characterized and compared in this study consist of the singly-enveloped nucleocapsids (SNPV) of Trichoplusia ni and the bundles of nucleocapsids common to a single envelope (MNPV) from Spodoptera frugiperda and Rachiplusia ou. The SNPV and MNPV DNAs are very similar in hydrodynamic properties and molecular weights. In addition, the NPV DNAs are similar in si...
متن کاملDevelopment of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses.
As supplements to classical chemical insecticides, two approaches to develop recombinant baculovirus insecticides are described. In one approach an insect-specific toxin is expressed leading to a dramatic reduction in time to death. In the second approach an insect juvenile hormone esterase is expressed which leads to a reduction in feeding. Modifications of the wildtype esterase led to viruses...
متن کاملVirus envelope acquisition of nuclear polyhedrosis virus in vitro.
There have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. While there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. Three possible ways have...
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ژورنال
عنوان ژورنال: Uirusu
سال: 1984
ISSN: 0042-6857,1884-3433
DOI: 10.2222/jsv.34.73